Electrical properties measurement of bio-particles using microfluidic chips

     This project aims at measuring electrical properties of biological particles such as Sperm, Ovum, Cell Organelles and etc. The extracted information then will be used for cell sorting and separation. Toward this goal different microfluidic chips with different channel sizes from micrometer to hundreds of micrometer has been fabricated using Polydimethylsiloxane called PDMS. Microfluidic chips are equipped with microelectrode structures, which need further optimization based on desired frequency range, cell properties and its environment. Microelectrodes have been fabricated on glass surface using advance sputtering techniques for metal deposition. Interaction of biological cells, electrodes and microfluidic channel and its contents changes the electrical capacitance of metal electrodes. An electronic now, can be used to measure the changes using transimpedance techniques. Finally based on measured impedance spectrum of the electrode-cell-microfluidic channel it is possible to extract the Dielectric Spectrum of the desired biological cell, which reflects directly cellular and subcellular properties, those which have vestigial effect in the desired frequency range.